This paper presents a new kind of trigonometric spline wavelet on the interval on the basis of trigonometric spline function. The proposed method has moreadvantages than any other traditional methods, such assymmetry and anti-symmetry-linear phases or generalizedlinear phases, good time-frequency locality, short support, semi-orthogonal. In the numerical experiment, thetrigonometric spline wavelet is applied to Electrocardiogram (ECG) detection. It shows stronger capability ofgetting more concentrated information in singular positionthan other wavelets. Therefore, the accuracy of detectionis improved greatly.