Portable System for Rapid Field Test of CEA in Human Serum Based on Chemiluminescence Enzyme Immunoassay

LIU Juntao; QU Shuxue; LUO Jinping; WANG Bin; CAI Xinxia

doi:10.1049/cje.2016.10.010

Volume 26 Issue 1

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Article Navigation > Chinese Journal of Electronics > 2017 > 26(1): 152-155

LIU Juntao, QU Shuxue, LUO Jinping, et al., “Portable System for Rapid Field Test of CEA in Human Serum Based on Chemiluminescence Enzyme Immunoassay,” Chinese Journal of Electronics, vol. 26, no. 1, pp. 152-155, 2017, doi: 10.1049/cje.2016.10.010

Citation:

LIU Juntao, QU Shuxue, LUO Jinping, et al., “Portable System for Rapid Field Test of CEA in Human Serum Based on Chemiluminescence Enzyme Immunoassay,” Chinese Journal of Electronics, vol. 26, no. 1, pp. 152-155, 2017, doi: 10.1049/cje.2016.10.010

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Portable System for Rapid Field Test of CEA in Human Serum Based on Chemiluminescence Enzyme Immunoassay

doi: 10.1049/cje.2016.10.010

LIU Juntao^1,2,
QU Shuxue^1,2,
LUO Jinping^1,2,
WANG Bin^1,2,
CAI Xinxia^{1,2
,}

1.
State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China;
2.
University of Chinese Academy of Sciences, Beijing 100190, China

Funds: This work is supported by the National Basic Research Program of China (No.2014CB744600), the National Natural Science Foundation of China (No.61527815, No.61471342), the Key Programs of the Chinese Academy of Sciences (No.KJZD-EW-L11-2), and the Bejing Municipal science and Technology Commission Program (No.Z141100000214002).

More Information

Corresponding author: CAI Xinxia (corresponding author) was born in Henan Province. She received the D.E. degree in electronic engineering from University of Glasgow. She is now a professor and Ph.D advisers of Institute of Electronics Chinese Academy of Sciences, she is also professor of University of Chinese Academy of Sciences. Her research interests include electronics science, biomedical engineering and biosensor. (Email:xxcai@mail.ie.ac.cn)
Received Date: 2015-01-06
Rev Recd Date: 2015-08-25
Publish Date: 2017-01-10

Abstract

Abstract

Carcinoembryonic antigen (CEA) is one of the most widely used tumor markers worldwide. A portable system was designed for rapid field specific test of CEA based on immunomagnetic separation and chemilu-minescence immunoassay, which consisted of chemiluminescent immunosensor and optical test instrument. The chemiluminescent immunosensor was based on immunomagnetic separation and chemiluminescence immunoassay, which utilized a immuno-sandwich scheme as-say with Horseradish peroxidase (HRP) labeled anti-CEA antibody and Immunomagnetic beads (IMBs). And the test instrument consisted of optical detection module and central processing and control system. The optical noise of this instrument was below 40 photons/s. The system could achieve a sample test in 30 minutes, and the detecting result showed a good liner relationship between the intensity of luminescence and concentration of CEA in the range from 1 to 1000ng/mL, with a correlation coefficient of 0.9858. Real human samples were also detected with this system in comparison with clinical results and a correlation coefficient of 0.9934 was obtained. The instrument is portable and easy to operate, which will provide potential application in cancer clinical test.
- Chemiluminescence immunoassay,
- Carcinoembryonic antigen,
- Rapid field test,
- Optical sensor,
- FPGA,
- NIOS II processor

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References(14)

References

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