LIU Juntao, QU Shuxue, LUO Jinping, WANG Bin, CAI Xinxia. Portable System for Rapid Field Test of CEA in Human Serum Based on Chemiluminescence Enzyme Immunoassay[J]. Chinese Journal of Electronics, 2017, 26(1): 152-155. doi: 10.1049/cje.2016.10.010
Citation: LIU Juntao, QU Shuxue, LUO Jinping, WANG Bin, CAI Xinxia. Portable System for Rapid Field Test of CEA in Human Serum Based on Chemiluminescence Enzyme Immunoassay[J]. Chinese Journal of Electronics, 2017, 26(1): 152-155. doi: 10.1049/cje.2016.10.010

Portable System for Rapid Field Test of CEA in Human Serum Based on Chemiluminescence Enzyme Immunoassay

doi: 10.1049/cje.2016.10.010
Funds:  This work is supported by the National Basic Research Program of China (No.2014CB744600), the National Natural Science Foundation of China (No.61527815, No.61471342), the Key Programs of the Chinese Academy of Sciences (No.KJZD-EW-L11-2), and the Bejing Municipal science and Technology Commission Program (No.Z141100000214002).
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  • Corresponding author: CAI Xinxia (corresponding author) was born in Henan Province. She received the D.E. degree in electronic engineering from University of Glasgow. She is now a professor and Ph.D advisers of Institute of Electronics Chinese Academy of Sciences, she is also professor of University of Chinese Academy of Sciences. Her research interests include electronics science, biomedical engineering and biosensor. (Email:xxcai@mail.ie.ac.cn)
  • Received Date: 2015-01-06
  • Rev Recd Date: 2015-08-25
  • Publish Date: 2017-01-10
  • Carcinoembryonic antigen (CEA) is one of the most widely used tumor markers worldwide. A portable system was designed for rapid field specific test of CEA based on immunomagnetic separation and chemilu-minescence immunoassay, which consisted of chemiluminescent immunosensor and optical test instrument. The chemiluminescent immunosensor was based on immunomagnetic separation and chemiluminescence immunoassay, which utilized a immuno-sandwich scheme as-say with Horseradish peroxidase (HRP) labeled anti-CEA antibody and Immunomagnetic beads (IMBs). And the test instrument consisted of optical detection module and central processing and control system. The optical noise of this instrument was below 40 photons/s. The system could achieve a sample test in 30 minutes, and the detecting result showed a good liner relationship between the intensity of luminescence and concentration of CEA in the range from 1 to 1000ng/mL, with a correlation coefficient of 0.9858. Real human samples were also detected with this system in comparison with clinical results and a correlation coefficient of 0.9934 was obtained. The instrument is portable and easy to operate, which will provide potential application in cancer clinical test.
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  • Bray F, Jemal A, Grey N, et al., "Global cancer transitions according to the Human Development Index (2008-2030):A population-based study", The Lancet Oncology, Vol.13, No.8, pp.790-801, 2012.
    S. Hundt, U. Haug and H. Brenner, "Blood markers for early detection of colorectal cancer:A systematic review", Cancer Epidemiology, Biomarkers and Prevention, Vol.16, No.10, pp.1935-1953, 2007.
    P. Li, B. Zhang and T. Cui, "Towards intrinsic graphene biosensor:A label-free, suspended single crystalline graphene sensor for multiplex lung cancer tumormarkers detection", Biosensors and Bioelectronics, Vol.72, No.15, pp.168-174, 2015.
    T. Hsu, "Unusual elevation of CEA in a patient with history of colon cancer", Japanese Journal of Clinical Oncology, Vol.32, No.6, pp.811-813, 2006.
    J. Michael, "Duffy carcinoembryonic antigen as a marker for colorectal cancer:Is it glinically useful", Clinical Chemistry., Vol.47, No.4, pp.624-630, 2001.
    S. Vingerhoedt, E. Hauben, R. Hermans, et al., "Elevated carcinoembryonic antigen tumour marker caused by head and neck cancer:A case report and literature study", Cancer/Radiothrapie, Vol.19, No.2, pp.106-110, 2015.
    T.C. Candido, S.H. Perri, T. Gerzoschkwitz, et al., "Comparative evaluation of enzyme-linked immunosorbent assay based on crude and purified antigen in the diagnosis of canine visceral leishmaniasis in symptomatic and oligosymptomatic dogs", Veterinary Parasitology, Vol.157, No.3-4, pp.175-181, 2008.
    E.W. Goertzen, K.E. Lloyd, J.W. Spears, et al., "Validation of a human double-antibody radioimmunoassay kit for measuring equine C-peptide", Journal of Equine Veterinary Science, Vol.35, No.5, pp.416-421, 2015.
    R. O. Matthew, W. Clarke, A. Zimlich, et al., "Optimization and development of a high-performance liquid chromatography based one-site immunometric assay with chemiluminescence detection", Analytica Chimica Acta, Vol.430, No.1, pp.37-50, 2012.
    T.K. Szegi, "Immunoluminometric detection of human procalcitonin", Journal of Biochemical and Biophysical Methods, Vol.53, No.1-3, pp.157-164, 2012.
    J.H. Granger, M.C. Granger, M.A. Firpo, et al., "Toward development of a surface-enhanced Raman scattering (SERS)-based cancer diagnostic immunoassay panel", Analyst, Vol.138, No.2, pp.1935-1953, 2013.
    J.Y. Hou, T.C. Liu, G.F. Lin, et al., "Development of an immunomagnetic bead-based time-resolved fluorescence immunoassay for rapid determination of levels of carcinoembryonic antigen in human serum", Analytica Chimica Acta, Vol.13, No.734, pp.93-98, 2012.
    S.X. Qu, J.T. Liu, J.P. Luo, et al., "BA rapid and highly sensitive portable chemiluminescent immunosensor of carcinoembryonic antigen based on immunomagnetic separation in human serum", Analytica Chimica Acta, Vol.766, No.5, pp.94-97, 2013.
    J.P. Luo, S.X. Qu, J.T. Liu, et al., "Preliminary characteristic of a portable optical biosensor for rapid detection of Cyfra21-1 based on chemiluminescence immunoassay using biofunctionlized magnetic nanocomposites", Chinese Science Bulletin, Vol.58, No.16, pp.1-3, 2013.
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